Kromasil^® Chiral

Works all the way

Kromasil^® products are well known for their ability to work along the whole spectrum from analytical to industrial scale chromatography. Kromasil^® AmyCoat and CelluCoat are no exception.

Simplifies method development

With particle sizes from 3 μm to 25 μm giving identical selectivity, Kromasil^® AmyCoat and CelluCoat make it easy to scale up while retaining excellent performance. As for all Kromasil^® products, the user can perform the required method development in analytical scale columns and then scale up to a larger column. For example, 3 μm particles in an analytical scale column can be scaled to a larger column packed with 10 μm particles. If the initial goal is to scale up the process, an analytical column packed with 10 μm particles can be used right from the start.

Easy to scale up

• Conditions
• Columns: Kromasil 3-CelluCoat 4.6 × 150 mm
•   Kromasil 5-CelluCoat 4.6 × 150 mm
•   Kromasil 10-CelluCoat 4.6 × 150 mm
• Substance: trans-stilbene oxide
• Mobile phase: heptane / 2-propanol (90/10)
• Flow rates: 0.5 ml/min
• Temperature: 25 °C
• Detection: UV @ 229 nm

Analytical scale

Preparative scale

• Analytical conditions
• Column size: 4.6 × 150 mm
• Flow rate: 0.5 ml/min
•  

• Prep conditions
• DAC system: NovaSep Pack-n-Sep, 50 mm i.d.
• Bed length: 135 mm
• Flow rate: 60 ml/min

• Common conditions
• Stationary phase: Kromasil 10-AmyCoat
• Mobile phase: heptane / 2-propanol (90/10)
• Substance: trifluoro-anthrylethanol
• Temperature: 20 °C
• Detection: UV @ 254 nm

Analytical scale

Preparative scale

• Analytical conditions
• Column size: 4.6 × 150 mm
• Flow rate: 0.5 ml/min
•  

• Prep conditions
• DAC system: NovaSep Pack-n-Sep, 50 mm i.d.
• Bed length: 132 mm
• Flow rate: 60 ml/min

• Common conditions
• Stationary phase: Kromasil 10-CelluCoat
• Mobile phase: heptane / 2-propanol (90/10)
• Substance: trifluoro-anthrylethanol
• Temperature: 20 °C
• Detection: UV @ 254 nm

Mechanically strong

Mechanical strength is an important product lifetime parameter. Kromasil^® AmyCoat and CelluCoat have mechanically strong spherical silica, which withstands repeated cycles of packing. The test was designed to exert greater than normal mechanical stress on the chiral stationary phases, and is performed at a packing pressure above the maximum 50 bar recommended by the manufacturer of Chiralcel OD and Chiralpak AD.

Loadability

Analytical injection (Chiralcel OD)

Preparative injection (Chiralcel OD)

Analytical injection (Kromasil^® CelluCoat)

Preparative injection (Kromasil^® CelluCoat)

• Common conditions
• Columns: Kromasil^® 10-CelluCoat 4.6 × 250 mm
•   Daicel Chiralcel OD (10 μm) 4.6 × 250 mm
• Mobile phase: heptane / 2-propanol / TFA (90/10/0.1)
• Substance: naproxen
• Temperature: 25 °C
• Flow rate: 0.5 ml/min

• Analytical conditions
• Sample load: 20 μg
•  

• Prep conditions
• Sample load: 5 mg
• Fraction size: 0.5 min

Fully back-integrated

Nouryon manufactures the super wide pore silica for Kromasil^® polysaccharide products and performs all subsequent steps leading to the final product. all products are fully traceable.

Every manufacturing step is ensured through Nouryon’s detailed quality system, and the final product is never released until it has passed a rigorous quality control test sequence.