Kromasil^® Chiral

Makes everyday work so much easier

Kromasil^® AmyCoat and CelluCoat allow the user to perform method development without interference from restrictive parameters such as pressure limits, equilibration times and long-term performance.

The economy of chiral chromatography

The lack of restrictions on various parameters makes method development particularly user-friendly. one well-known restriction for coated polysaccharide phases is the general pressure limit over the bed. Kromasil^® AmyCoat and CelluCoat withstand flow rates equivalent to pressures of up to 400 bar – which is about the limit for a standard HPlC system itself. This allows users to run chiral chromatography very fast.

Stable performance

When it comes to stability, Kromasil^® AmyCoat and CelluCoat are compatible with normal, polar organic and reversed mobile phases. switching between compatible normal to polar organic mobile phases does not lead to any reduction in performance and there is no need for solvent dedicated columns.

Stable performance – Freedom to switch solvents

• Conditions
• Column: Kromasil 3-AmyCoat 4.6 × 150 mm
• Substance: trans-stilbene oxide
• Mobile phase: heptane / 2-propanol (90/10)

•  
• Flow rate: 1 ml/min
• Temperature: 25 °C
• Detection: UV @ 229 nm

• Conditions
• Column: Kromasil 5-CelluCoat 4.6 × 250 mm
• Substance: trans-stilbene oxide
• Mobile phase: heptane / 2-propanol (90/10)
• Flow rates: 0.1 – 1.2 ml/min
• Temperature: 25 °C

Stable performance – No pressure limits

• Conditions
• Column: Kromasil 3-CelluCoat 4.6 × 50 mm
• Substance: trans-stilbene oxide
• Mobile phase: heptane / 2-propanol (90/10)
• Flow rates: 7 ml/min
• Temperature: 25 °C

Short equilibration times

Column equilibration is a time-consuming activity when running chiral chromatography. in general, long equilibration times are most pronounced when switching mobile phases containing basic additives to acidic additives or the other way around. The test with a Kromasil^® CelluCoat 3 μm column switching between two compatible mobile phases shows how short the needed equilibration times actually are.

• Common conditions
• Column: Kromasil^® 3-CelluCoat 4.6 × 150 mm
• Substance: trans-stilbene oxide
• Flow rate: 2 ml/min
• Temperature: 25 °C
• Detection: UV @ 229 nm

• Conditions 1
• Mobile phase: heptane / 2-propanol (90/10)

• Conditions 2
• Mobile phase: ethanol

No memory effects

These two tests illustrate short equilibration times and additive switches for Kromasil^® AmyCoat and CelluCoat with absolutely no sign of memory effects.

No memory effects – Freedom to switch additives

• Common conditions
• Column: Kromasil 3-AmyCoat 4.6 × 150 mm
• Flow rate: 2 ml/min
• Temperature: 25 °C

• Conditions 1
• Substance: carbinoxamine
• Mobile phase: heptane / 2-propanol / DEA (90/10/0.1)
• Detection: UV @ 228 nm

• Conditions 2
• Substance: ketoprofen
• Mobile phase: heptane / 2-propanol / TFA (90/10/0.1)
• Detection: UV @ 250 nm

• Common conditions
• Column: Kromasil 3-CelluCoat 4.6 × 150 mm
• Flow rate: 1 ml/min
• Temperature: 25 °C

• Conditions 1
• Substance: naproxene
• Mobile phase: heptane / 2-propanol / TFA (90/10/0.1)
• Detection: UV @ 226 nm

• Conditions 2
• Substance: Tröger’s base
• Mobile phase: heptane / 2-propanol / DEA (90/10/0.1)
• Detection: UV @ 220 nm

Reverse phase compatibility

Many chiral separations are run under normal phase conditions. Sometimes, though, reversed-phase (RP) conditions are required to achieve proper separation. While it is possible to convert Kromasil^® AmyCoat or CelluCoat columns to run under RP-mode, it might be quicker and more efficient to use a column initially conditioned for RP-mode: Kromasil^® AmyCoat RP and CelluCoat RP.

Kromasil 3-AmyCoat

Kromasil 3-AmyCoat RP

• NP conditions
• Column: Kromasil 3-AmyCoat 4.6 × 150 mm
• Mobile phase: heptane / 2-propanol (90/10)
• Flow rate: 0.25 ml/min

• RP conditions
• Column: Kromasil 3-AmyCoat RP 4.6 × 150 mm
• Mobile phase: acetonitrile / water (40/60)
• Flow rate: 0.5 ml/min

• Common conditions
• Substance: 2-phenyl-2-butanol
• Temperature: 22 °C
• Detection: UV @ 254 nm