Kromasil^® Chiral

Fast and easy method development

To speed up and simplify method development, Nouryon has removed some of the restrictions for coated polysaccharide phases. In analytical scale chromatography, 3 μm particles and the absence of pressure limits allow fast chromatography with good separation results.

Good results

Kromasil^® AmyCoat and CelluCoat show excellent enantioselectivity for many racemates. In the application section of this guide, there are many chiral applications showing the performance levels scientists can expect.

By having access to 3 μm particles, higher plate count and resolution can be expected for analytical chromatography. Combined with excellent selectivity, this facilitates the separation of enantiomers.

Saving time

With Kromasil^® AmyCoat and CelluCoat, users get better results faster. Thanks to the absence of pressure limits, analytical chromatography can be run at very high flow rates and thereby save time.

Selectivity and resolution comparison
– Kromasil^® AmyCoat 3 μm and Chiralpak AD-H (5 μm)

• Conditions
• Columns: Kromasil 3-AmyCoat 4.6 × 150 mm
•   Chiralpak AD-H (5 μm), 4.6 × 150 mm
• Mobile phase: heptane / 2-propanol / DEA (90/10/0.1)
• Substance: carbinoxamine

•  
• Flow rate: 0.5 ml/min
• Temperature: 22 °C
• Detection: UV @ 226 nm
•  

	α		Rs
	AmyCoat 3 μm	Chiralpak AD-H*	AmyCoat 3 μm	Chiralpak AD-H*
Ambucetamide	1.4	1.4	4.8	4.2
Carbinoxamine	1.5	1.5	7.3	5.8
Ketoprofen	1.4	1.3	4.6	4.3
Naproxen	1.2	1.2	3.4	3.1
Oxamniquine	1.2	1.2	3.3	3.1
Proglumide	2.7	2.8	11.8	9.0
Sulindac	1.3	1.3	4.8	3.9

(* 5 μm)

Selectivity and resolution comparison
– Kromasil^® CelluCoat 3 μm and Chiralcel OD-H (5 μm)

• Conditions
• Columns: Kromasil 3-CelluCoat 4.6 × 150 mm
•   Chiralcel OD-H (5 μm), 4.6 × 150 mm
• Mobile phase: heptane / 2-propanol (90/10)
• Substance: trans-stilbene oxide

•  
• Flow rate: 1 ml/min
• Temperature: 25 °C
• Detection: UV @ 229 nm
•  

	α		Rs
	CelluCoat 3 μm	Chiralcel OD-H*	CelluCoat 3 μm	Chiralcel OD-H*
trans-Stilbene oxide	2.4	2	11.5	8.3
Benzoin	1.5	1.5	6.5	5.7
Trifluoro-anthrylethanol	2.9	2.9	11.6	11
Trögers base	1.41	1.4	3.2	2.7
Oxprenolol	5.6	5.5	14.7	15.1
Naproxen	1.2	1.2	2.6	2.2
Proglumide	1.8	2	4.7	3.2

(* 5 μm)

Difference in resolution–Kromasil^® AmyCoat 3 μm vs. 5 μm

• Conditions
• Columns: Kromasil 5-AmyCoat 4.6 × 150 mm
•   Kromasil 3-AmyCoat 4.6 × 150 mm
• Mobile phase: heptane / 2-propanol (95/5)
• Substance: trans-2-phenyl-1-cyclohexanol
• Flow rate: 1 ml/min
• Temperature: 25 °C
• Detection: UV @ 220 nm

Fast analytical chromatography

• Conditions
• Columns: Kromasil 5-CelluCoat 4.6 × 250 mm
•   Kromasil 3-CelluCoat 4.6 × 150 mm
•   Kromasil 3-CelluCoat 4.6 × 50 mm
• Substance: trans-stilbene oxide

•  
• Mobile phase: heptane / 2-propanol (90/10)
• Flow rate: 1 - 7 ml/min (see figures)
• Temperature: 25 °C
• Detection: UV @ 229 nm