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The Kromasil® Classic 300 Å family of products is designed to be the perfect choice for proteins and biomolecules larger than 8–10 kDa. This 300 Å material has a narrow pore size distribution that ensures good mass transfer for larger molecules, resulting in narrow peaks and no size-exclusion effects. The figures below show FE-SEM studies of Kromasil® 300 Å, indicating a very regular pore structure, with no voids nor dense clusters.

FE-SEM cut through
FE-SEM picture of a cut through a Kromasil® 300 Å particle at 5 000 x magnification.
FE-SEM cut through
FE-SEM picture of a cut through Kromasil® 300 Å particle at 35 000 x magnification, showing both the outer surface and the fracture through the particle.

Tryptic digest of bovine serum albumin (BSA)

Tryptic digest of bovine serum albumin (BSA)
A common test for RP packings designed for the separation of biological materials is to run a tryptic digest of BSA. The digest contains fragments of various sizes, and the separation of these into individual peaks is good evidence of the power of resolution.
  • Conditions
  • Column: Kromasil 300-5-C4 4.6 × 250 mm
  • Mobile phase A: acetonitrile / water / TFA (4/96/0.085)
  • Mobile phase B: acetonitrile / water / TFA (90/10/0.1)
  • Gradient: 0 min: 4%, 5 min: 4%, 80 min: 40% acetonitrile
  • Flow rate: 1.0 ml/min
  • Temperature: 22 °C
  • Detection: UV @ 215 nm

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